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KMID : 0385019990150030243
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Korean Journal of Laboratory Animal Science
1999 Volume.15 No. 3 p.243 ~ p.249
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Analysis of the Phenotype in the hCG(human Chorionic Gonadotropin) β-subunit Transgenic Mouse
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최재훈/Choi, Jae Hoon
정의배/윤영원/한동운/오구택/이병재/장규태/Jeung, Eui Bae/Yun, Young Won/Han, Dong Un/Oh, Ku Taek/Lee, Byeung Jae/Chang, Kyu Tae
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Abstract
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Chorionic gonadotropin(CG) is a member of superfamily of glycoprotein hormones including luteinizing hormone (LH), follicle-stimulating hormone (FSH) and thyroid-stimulating hormone (TSH). The four hormones are heterodimer with a common α subunit and unique β subunint. While LH, FSH, and TSH are synthesized in the pituitary, but CG is produced in the placenta. The human LHβ and CGβ subunits are structurally and functionally very similar. It is difficult to study the function and regulation of hCG hormone due to absence of mouse model. Therefore, the transgenic mouse model with hCGβ-subunit gene was suitable for studying the temporal gene expression in the placenta during pregnancy. Unexpectively, this mouse showed a particular phenotype in terms of increase of the body weight. To characterize the phenotype observed in the CGβ transgenic mice, these mice were analyzed with the biomedical and molecular biological techniques.
The confirmation of transgene in offsprings was determined by polymerase chain reaction (PCR) with genomic DNA from their tails. All transgenic mice allocated in these experiments were homozygous during the period from 26 to 56 weeks old. The mice were non-transgenic from the same littermate as a control. Male and female mice were used in equal proportion. The results were as followings; 1. The body weight of CGβ transgenic mice was significantly increased (p$lt;0.05) from 28 weeks and highest at the age of 56 weeks. 2. There were no typical differences in the histological observation of the liver, kidney and pancreas except adipose tissue in the CGβ transgenic mice compared non-transgenic mice. 3. The epididymal fat pad was hypertropied in the CGβ transgenic mice after 50 weeks old. 4. The CGβ 1, 2 gene in the transgenic mice was expressed in the brain and significantly (p$lt;0.05) increased in estrus stage during the estrus cycle. 5. Blood chemical analysis showed the significant increase in the values of glucose (p$lt;0.05 with n=5), total cholesterol (p$lt;0.05 with n=5), but not triglyceride (p$lt;0.05 with n=5) in the blood of CGβ transgenic mice, respectively.
These results have shown that the CGβ transgenic could be applicable as a new obesity model based on increase in body weight and abdominal fat mass. The biochemical and physiological mechanisms were remained to study using the CGβ transgenic mice.
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KEYWORD
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